Electric-assisted bicycles (Pedelecs) offer a promising pathway toward sustainable mobility by combining the convenience of motorized travel with the environmental benefits of cycling. This study proposes to examine how access to Pedelecs—compared to conventional bicycles—affects the reduction of single-occupancy car trips in Germany. Using a large, representative dataset of over >100,000 licensed car drivers, the research will analyze mode choice behaviors across diverse socio-economic and geographic contexts. A central focus will be the comparative impact of Pedelec and conventional bicycle use on car-mode substitution. The study will also investigate how these effects vary between urban and rural areas, where car dependency and infrastructure differ substantially. By distinguishing the relative contributions of both bicycle types, this research aims to inform targeted policy interventions that maximize the potential of active mobility modes to reduce car reliance, emissions, and congestion.