Tear film breakup (TBU) is thought to be a major contributor to dry eye syndrome, which afflicts millions worldwide. Although clinicians can easily conduct fluorescence imaging during patient visits and observe what appears to be TBU, there are no widely accepted objective characterizations of TBU, let alone software that can automatically detect it. Julia is being used to detect the corneal region in fluorescence videos, collect regional intensity, and cluster regions based on TBU likelihood.