Polyethylene (PE) is the most commonly used polymer for plastic production, causing an environmental problem nowadays. Bacteria play an important role in the biodegradation of PE, which could be a possible way to solve this problem. However, research into the biodegradation of HDPE, the linear form of PE, is less well studied than that of LDPE, the branch form. This study aims to isolate and identify bacteria capable of HDPE degradation from the soil by providing different HDPE powders as their carbon source (PE-01, PE-02, PE-03, PE-04 and PE-05). Seventeen isolates that grew in the cultures containing PE powder were identified using 16S rRNA gene sequencing. Four isolates are belonging to the genus Acinetobacter whereas thirteen isolates are a member of Pseudomonas genus. The isolates of Acinetobacter sp. were isolated from almost all PE culture but not from only PE-03 culture. For PE degradation analysis, the results from LC-MS showed that the pattern of substrates in all cultures changed over time which could cause by bacterial activity to degrade PE. GPC analysis showed an increase in the Mn and Mw of PE in PE-01, PE-04, PE-05 cultures. In addition, HDPE commercial bags were used to investigate the potential of bacteria in soil capable of PE biodegradation by growing in LB or M9 media. After seven days of incubation, the weight of the films inoculated with soil inoculum reduced approximately 1.6% in LB while there was no reduction in M9. The PE films incubated with soil inoculum showed a significant change on the surface such as cracking examined by SEM analysis. It is hoped that this research will contribute to the growing body of knowledge about HDPE-degrading bacteria and their ability to degrade HDPE in a short incubation time.